PERSISTENT PHA-RESPONSIVE POPULATION IN MOUSE THYMUS .1. CHARACTERIZATION OF POPULATION

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (4) , 383-394
Abstract
Using an in vitro culture technique, mouse thymus graft cells were co-cultured with peripheral blood lymphocytes in the presence of phytohemagglutinin (PHA). The persisten PHA-responsive thymus graft population was able to respond to other T[thymus-derived]-cell mitogens (Con [concanavalin] A, pokeweed mitogen, staphylococcal enterotoxin B), but not Escherichia coli lipopolysaccharide, a known B[bone marrow-derived]-cell mitogen. The percentage of persistent PHA-responsive cells did not alter during 5 days in culture and was relatively unaffected by hydrocortisone or anti-lymphocyte serum treatment in vivo. In allogeneic thymus grafts (AKR .fwdarw. CBA), persistent PHA-responsive cells could be demonstrated which were destroyed when incubated with CBA anti-.theta.AKR serum and complement. When thymus graft cells were allowed to sediment on a 0.2-2% BSA [bovine serum albumin] gradient, the PHA-responsive population sedimented faster than the bulk of thymus graft cells. Some separation could be obtained on this gradient between the persistent and non-persistent PHA-responsive cell populations.

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