Peroxidases in tobacco abscission zone tissue. VI. Ultrastructural localization in plasmodesmata during ethylene-induced abscission.

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Abstract
The ultrastructural localization of peroxidases, during ethylene-induced abscission, in flower pedicels of Nicotiana tabacum L. ''Little Turkish'', was investigated. Peroxidase activity was localized in the plasmodesmata of abscission zone tissue that was fixed in glutaraldehyde and subsequently incubated in diaminobenzidine (DAB) medium with post-fixation in osmium tetroxide. Preincubation of tissue in potassium cyanide, prior to incubation in DAB medium containing the inhibitor, demonstrates a significant reduction in DAB staining. Incubation of tissue in aminotriazole (an inhibitor of catalase activity), prior to incubation in DAB medium containing the inhibitor, does not inhibit the peroxidase activity in plasmodesmata incubated in DAB medium. Aminothiazole does inhibit catalase present in the microbodies of DAB incubated tissue. Proliferating plasmodesmata, in response to ethylene treatment, exhibit enhanced peroxidase activity and allow for the increased mobilization of peroxidase to the middle lamellar areas of the cell walls of abscising cells.

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