COMBINED USE OF RFLP AND PCR‐ASO TYPING FOR HLA‐DR‐Dw AND DQw TYPING
- 1 February 1991
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 18 (1-2) , 125-138
- https://doi.org/10.1111/j.1744-313x.1991.tb00012.x
Abstract
Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.Keywords
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