Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking
Top Cited Papers
- 17 October 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 302 (5644) , 442-445
- https://doi.org/10.1126/science.1088525
Abstract
Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.Keywords
This publication has 23 references indexed in Scilit:
- Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in VivoScience, 2003
- The role of receptor diffusion in the organization of the postsynaptic membraneNature Reviews Neuroscience, 2003
- In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid MicellesScience, 2002
- Regulation of AMPA receptor lateral movementsNature, 2002
- Precise Nanometer Localization Analysis for Individual Fluorescent ProbesPublished by Elsevier ,2002
- Strychnine-Blocked Glycine Receptor Is Removed from Synapses by a Shift in Insertion/Degradation EquilibriumMolecular and Cellular Neuroscience, 2002
- Single-Molecule Analysis of Chemotactic Signaling in Dictyostelium CellsScience, 2001
- Predicted structure of the extracellular region of ligand-gated ion-channel receptors shows SH2-like and SH3-like domains forming the ligand-binding siteProtein Science, 1997
- Fluorescence intermittency in single cadmium selenide nanocrystalsNature, 1996
- Distribution of glycine receptors at central synapses: an immunoelectron microscopy study.The Journal of cell biology, 1985