Electron Microscopy of the Peroxidase in the Granular Leucocytes of Rat Bone Marrow

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Abstract
Peroxidase reaction was performed on the frozen section of the glutaraldehyde-fixed rat bone marrow. The section was postfixed with OsO4 and prepared for electron microscopy. Peroxidase reaction was demonstrated on the neutrophilic and eosinophilic granules but not on the basophilic granules. Peroxidase reaction was stronger in the peripheral zone of each granule and was negative in the central crystalloid. In a certain promyelocytes of neutrophiles as well as eosinophiles, the granular endoplasmic reticulum and Golgi apparatus showed a positive reaction. The reaction was stronger in the eosinophiles. The possibility to regard granulocytes as unicellular glandular cell was discussed.