Callus Culture of Sainfoin (Onobrychis viciifolia) and Plant Regeneration through Somatic Embryogenesis
- 1 September 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 60 (3) , 309-313
- https://doi.org/10.1093/oxfordjournals.aob.a087449
Abstract
Callus of sainfoin (Onobrychis viciifolia Scop.) was initiated from stem and root explants which were obtained from seedlings growing in vitro, on Linsmaier Skoog (LS) medium supplemented with 1 mg l−1 2, 4-D and 1 mg l−1 BA or only 1 mg l−1 BA, and the Vacin and Went medium without hormones. Somatic embryos were formed on LS medium containing 1 m l−1 BA. Embryos developed into complete plants on filter paper saturated with hormone-free LS medium.Keywords
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