Effects of Age and Growth Regulators on Serially Propagated Digitalis lanata Leaf and Root Cultures

Publisher Website
Google Scholar
Abstract
The cardenolide TLC and GLC patterns from leaf and root culture extracts were similar to those obtained from the plant. Leaf cultures at 8 and 12 wk old contained more primary cardiac glycosides (Lanatoside A, B, C) than 4 wk old leaf cultures. As high as 44 mg% dry wt of digoxin, as determined by RIA [radioimmunoassay], was present in 12 wk old leaf cultures. The concentration of digoxin increased in both leaf and root cultures when treated with either GA or mefluidide and leaf organ cultures elongated with GA treatment.