The Oxidation of 14C-labelled Glucose by Chlorella vulgaris
- 1 January 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 26 (1) , 71-82
- https://doi.org/10.1093/oxfordjournals.aob.a083777
Abstract
Glucose, either uniformly labelled with14C, or specifically labelled in the I, 2, or 6 position, was added to C. vulgaris. Radio-active carbon dioxide was produced initially ten times faster from glucose-I-14C than from glucose-6-14C. This difference was found with carbohydrate-starved cultures, exponentially growing cultures, and cultures assimilating ammonia or nitrate rapidly. A similar difference was also found with C. pyrenoidosa and Ankistrodesmus. 37 per cent. of the 14C added as glucose-1-14C to exponentially growing cells was recovered as carbon dioxide but generally the recovery was less than this. Only 5 per cent. of 14C added as glucose-6-14C was recovered as carbon dioxide. The specific activity of the carbon dioxide produced was considerably lower than that of the carbon in the added glucose.This publication has 1 reference indexed in Scilit:
- The Use of Glucose-C14 for the Evaluation of the Pathways of Glucose MetabolismJournal of Biological Chemistry, 1960