Digoxigenin as a probe label forin situ hybridization on skeletal tissues
- 1 September 1991
- journal article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 23 (9) , 415-418
- https://doi.org/10.1007/bf01042298
Abstract
Non-specific staining was encountered using digoxigenin-labelled cDNA probes forin situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.Keywords
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