Human hybridomas from patients with malignant disease
Open Access
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 47 (1) , 135-145
- https://doi.org/10.1038/bjc.1983.16
Abstract
Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants.Keywords
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