Bromodomain factor 1 (Bdf1) protein interacts with histones
- 4 May 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 496 (1) , 31-35
- https://doi.org/10.1016/s0014-5793(01)02397-3
Abstract
Using a yeast two‐hybrid assay we detected an interaction between the N‐terminal region of histone H4 (amino acids 1–59) and a fragment of the bromodomain factor 1 protein (Bdf1p) (amino acids 304–571) that includes one of the two bromodomains of this protein. No interaction was observed using fragments of histone H4 sequence smaller than the first 59 amino acids. Recombinant Bdf1p (rBdf1p) demonstrates binding affinity for histones H4 and H3 but not H2A and H2B in vitro. Moreover, rBdf1p is able to bind histones H3 and H4 having different degrees of acetylation. Finally, we have not detected histone acetyltransferase activity associated with Bdf1p.Keywords
This publication has 32 references indexed in Scilit:
- Mediator–Nucleosome InteractionMolecular Cell, 2000
- Structure and Function of a Human TAF II 250 Double Bromodomain ModuleScience, 2000
- The language of covalent histone modificationsNature, 2000
- Sas3 Is a Histone Acetyltransferase and Requires a Zinc Finger MotifBiochemical and Biophysical Research Communications, 1999
- The bromodomain of Gcn5p interacts in vitro with specific residues in the N terminus of histone H4 1 1Edited by T. RichmondJournal of Molecular Biology, 1999
- The bromodomain revisitedTrends in Biochemical Sciences, 1997
- A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1ANature, 1996
- Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.Genes & Development, 1996
- Identification of a Gene Encoding a Yeast Histone H4 AcetyltransferasePublished by Elsevier ,1995
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976