μ-Opioid Peptides Inhibit Thalamic Neurons

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Abstract
Opioidergic inhibition of neurons in the centrolateral nucleus of the thalamus was investigated using anin vitrothalamic slice preparation from young rats. The μ-opioid receptor agonistd-Ala2,N-Me-Phe4,glycinol5-enkephalin (DAMGO) evoked a hyperpolarization and decrease in input resistance that was reversible, concentration-dependent, and persisted in the presence of tetrodotoxin. Application of the specific μ-receptor antagonist Cys2,Tyr3,Orn5,Pen7-amide blocked this response. The respective δ- and κ-opioid receptor agonists, (d-Pen2,d-Pen5)-enkephalin and (±)-trans-U-50488 methanesulfonate had no effect. Voltage-clamp experiments showed that DAMGO activated an inwardly rectifying potassium conductance (GKIR) characterized by rectification at hyperpolarized potentials that increased in elevated extracellular potassium concentrations, a complete block by Ba2+(1 mm), and a voltage-dependent block by Cs+. The extent of μ-opioid inhibition in other thalamic nuclei was then investigated. Widespread inhibition similar to that seen in the centrolateral nucleus was observed in a number of sensory, motor, intralaminar, and midline nuclei. Our results suggest that the net action of opioids would depend on their source: exogenous (systemically administered) opiates inhibiting the entire thalamus and favoring the shift of cell firing from tonic to bursting mode; and endogenously released opioids acting on specific thalamic nuclei, their release depending on the origin of the presynaptic input.