Biochemical-Genetic Characterization and Distribution of OXA-22, a Chromosomal and Inducible Class D β-Lactamase fromRalstonia(Pseudomonas)pickettii
Open Access
- 1 August 2000
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 44 (8) , 2201-2204
- https://doi.org/10.1128/aac.44.8.2201-2204.2000
Abstract
From genomic DNA ofRalstonia pickettiiisolate PIC-1, a β-lactamase gene was cloned that encodes the oxacillinase OXA-22. It differs from known oxacillinases, being most closely related to OXA-9 (38% amino acid identity). The hydrolytic spectrum of OXA-22 is limited mostly to benzylpenicillin, cloxacillin, and restricted-spectrum cephalosporins. OXA-22-like genes were identified as single chromosomal copies in five otherR. pickettiiclinical isolates. The expression of OXA-22-like β-lactamases was inducible inR. pickettii.Keywords
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