DEOXYRIBONUCLEIC ACID CYTOCHEMISTRY FOR AUTOMATED CYTOLOGY

Open Access

1 July 1974

journal article
research article
Published by SAGE Publications in Journal of Histochemistry & Cytochemistry

Vol. 22 (7) , 470-477
https://doi.org/10.1177/22.7.470

Abstract

Fluorescent staining for intracellular deoxyribonucleic acid content is being investigated as a means of making automated measurements quantitative and reliable. Ambiguities regarding the role of sulfur dioxide in Feulgen staining chemistry have been resolved showing that: (a) SO₂ does not covalently bind to the anilinium moieties of pararosaniline; (b) SO₂ does promote the binding of pararosaniline within the nuclei of hydrolyzed cells; (c) SO₂ also does not bind to dyes such as acriflavine used in fluorescent-"Feulgen" staining and (d) SO₂ is not required for strong, nucleus-specific acriflavine staining of nuclei of hydrolyzed cells. These findings permit simplifications of the usual fluorescent-"Feulgen" procedures. Attempts to use fluorescent intercalating dyes as vital stains for deoxyribonucleic acid content have been unsuccessful.

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