Lac UV5 transcription in vitro. Rate limitation subsequent to formation of an RNA polymerase-DNA complex
- 20 March 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (6) , 1063-1067
- https://doi.org/10.1021/bi00573a020
Abstract
The kinetics of transcription of lac UV5 mRNA using purified DNA restriction fragment as template was studied. This template, which contains only 203 base pairs, directs the formation of a 67-base lac mRNA with high specificity. The half-time for formation of a DNA-RNA polymerase complex is approximately 0.2 min. Upon addition of 200 .mu.M nucleoside triphosphates to this complex, RNA production proceeds with a half-time approximately 1 min. The rate-limiting step for lac UV5 mRNA production, under typical in vitro conditions, probably occurs subsequent to the formation of a promoter-specific complex. [Escherichia coli was used.].This publication has 4 references indexed in Scilit:
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