Effect of stage of cycle, sampling frequency and recovery of micro-organisms on total protein content of mare uterine flushings

Abstract
Mares with sound reproductive tracts were divided into 2 groups. In group I (n = 12), uteri were flushed once per estrous cycle during alternate cycles while in group II (n = 8) mares were flushed twice in a cycle for 2 contiguous cycles. Total protein concentrations and total recoverable protein of uterine flushings taken on day 3 of estrus and day 8 after ovulation in each of the 2 groups did not differ significantly. The length of estrus and diestrus was not affected by the flushing procedures. Total recoverable protein and total protein concentrations of flushings were higher at day 3 of estrus and day 8 and 15 after ovulation (P < 0.01) when a microorganism was isolated from the uterus before flushing.