Oxidative Phosphorylation in Germinating Lettuce Seeds

Abstract
The effect of thiourea and coumarin, in vivo and in vitro, on the phosphorylating activity of lettuce mitochondria was investigated, as well as the effect of coumarin on the P/O ratio. It was shown that both substances in vitro inhibit phosphorylation; while in vivo coumarin inhibits but thiourea under certain circumstances stimulates. Coumarin was also shown to decrease the P/O ratio and therefore may be considered as an uncoupler. The difficulties in considering these effects of the substances as a primary mechanism controlling germination are pointed out and discussed.

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