Studies in Protein Metabolism of the Amphibian Embryo. II. Free Amino Acids

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Abstract
The following free amino acids were identified by paper chromatog-raphy in ethanol extracts of zygotes, gastrulae, neurulae, and tailbud embryos of Rana pipiens and Hyla regilla: glutamic acid, serine, threonine, tyrosine, alanine, valine, phenylalanine, leucine (or isoleucine). In addition were found glutamine, several peptides, and several unidentified ninhydrin-positive substances and possibly, in Rana only, methionine sulfoxide. Rana and Hyla chromatograms were very similar. There were only a few minor changes as development proceeded. One expt. on Hyla revealed no major chromatographic difference between explants of dorsal lip and ventral ectoderm.