Detection of the tripeptide Tyr‐Gly‐Gly, a putative enkephalin metabolite in brain, using a sensitive radioimmunoassay
- 23 September 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 189 (2) , 325-328
- https://doi.org/10.1016/0014-5793(85)81049-8
Abstract
A sensitive and specific radioimmunoassay has been developed for YGG. The tripeptide was previously derivatised with p-benzoquinone to prepare the immunogen and the 125I tracer as well as in samples submitted to the RIA. The sensitivity is about 1 nM as compared with 8000 nM for underivatised YGG. Measurable amounts of endogenous YGG immunoreactivity, co-eluting in HPLC with authentic YGG, were detected in mouse striatal extracts.Keywords
This publication has 8 references indexed in Scilit:
- Enkephalinase from rat kidney. Purification, characterization, and study of substrate specificity.Journal of Biological Chemistry, 1984
- The enkephalinase inhibitor thiorphan shows antinociceptive activity in miceNature, 1980
- High-affinity enkephalin-degrading peptidase in brain is increased after morphineNature, 1978
- Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylglycolurilBiochemistry, 1978
- RADIOIMMUNOASSAY OF METHIONINE‐AND LEUCINE‐ENKEPHALINS IN REGIONS OF RAT BRAIN AND COMPARISON WITH ENDORPHINS ESTIMATED BY A RADIORECEPTOR ASSAYJournal of Neurochemistry, 1978
- Conjugation of p-Benzoquinone treated enzymes with antibodies and Fab fragmentsImmunochemistry, 1977
- The adsorption of enkephalin to porous polystyrene beads: A simple assay for enkephalin hydrolysisFEBS Letters, 1977
- Bestatin, an inhibitor of aminopeptidase B, produced by actinomycetes.The Journal of Antibiotics, 1976