The Inactivation of Rinderpest Virus by β-Propiolactone and Its Effect on Homologous Complement-Fixing and Neutralizing Antibodies
Open Access
- 1 October 1961
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 87 (4) , 464-467
- https://doi.org/10.4049/jimmunol.87.4.464
Abstract
Summary: Cattle inoculated with 5 × 104 lethal doses of Pendik rinderpest virus treated with 0.4% β-propiolactone (BPL) did not develop the disease and were susceptible to challenge. The same concentration of BPL also inactivated the Nakamura III strain of rinderpest. BPL in concentrations from 0.1 to 1.0% did not significantly alter the complement-fixing (CF) activity of anti-rinderpest rabbit serum. However, the titers as measured by agar gel diffusion (AD) were lowered. Rabbit anti-rinderpest serum treated with 0.4% BPL retained its virus neutralizing capacity when tested in rabbits. The CF and AD titers of Pendik virus were not altered after treatment with BPL in concentrations ranging from 0.1 to 1%.Keywords
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