Relative synthesis of cardiac contractile proteins. Evidence for synthesis from the same precursor pool
- 15 March 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 194 (3) , 673-678
- https://doi.org/10.1042/bj1940673
Abstract
The relative molar synthesis of cardiac contractile proteins has been measured in the perfused heart under control haemodynamic conditions. This synthesis, of myosin heavy chains, individual light chains (1 and 2), actin and tropomyosin, was determined from isolated guinea-pig hearts perfused for 3h simultaneously with constant specific radioactivities and concentrations of [3H]lysine and [3H]phenylalanine.The data strongly suggest that all of the proteins studied were synthesized from the same precursor pools of lysine and phenylalanine, since the ratio of the specific activities of the two labels was the same in all of the proteins. Measurement of molar synthesis of each contractile protein was the same with either labelled amino acid. Under control haemodynamic-perfusion conditions, the relative molar synthesis of the contractile proteins was actin greater than heavy chains greater than light chain 2 greater than light chain 1 greater than tropomyosin.This publication has 37 references indexed in Scilit:
- Early changes in myocardial protein synthesis in vivo in response to right ventricular pressure overload in the dogJournal of Molecular and Cellular Cardiology, 1979
- Action of triiodothyronine on the synthesis of rat ventricular myosin isoenzymesFEBS Letters, 1979
- Synthesis of myosin heavy and light chains in the afterloaded guinea pig right ventricleCardiovascular Research, 1978
- Protein synthesis in prolonged cardiac arrestJournal of Molecular and Cellular Cardiology, 1977
- Turnover of myosin heavy and light chains in cultured embryonic chick cardiac and skeletal muscleDevelopmental Biology, 1976
- Sampling of the leucine pool from the growing peptide chain: Difference in leucine specific activity of peptidyl-transfer RNA from free and membrane-bound polysomesJournal of Molecular Biology, 1975
- The effect of pressure or flow stress on right ventricular protein synthesis in the face of constant and restricted coronary perfusion.Journal of Clinical Investigation, 1975
- Myosin chains of myocardial tissue — I. Purification and immunological properties of myosin heavy chainsBiochemical and Biophysical Research Communications, 1973
- Ca2+-binding and Ca2+-sensitizing functions of cardiac native tropomyosin, troponin, and tropomyosinBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- Muscle fibrils: Solubilization and gel electrophoresisFEBS Letters, 1971