New Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV , Inserted in a Class 1 Integron
- 1 September 1998
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 42 (9) , 2221-2224
- https://doi.org/10.1128/aac.42.9.2221
Abstract
The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV , which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ ant (3′′)-Ia ], and sulfonamide resistance, sulI , were located downstream of dfrXV in a manner identical to that in pLMO229.Keywords
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