Emetine resistance in chinese hamster ovary cells is associated with an altered ribosomal protein S14 mRNA.

Publisher Website
Google Scholar
Abstract
Chinese hamster ovary cell mutants resistant to the translational inhibitor emetine (Emt B mutants) express a single genetically altered 40S ribosomal subunit protein, S14, that appears to account for their drug resistance. To determine whether Emt B mutations affect the structural gene for the S14 protein, mRNA were isolated from several wild-type and Emt B mutant clones. The mRNA were translated in a cell-free system derived from wheat germ, and the biosynthesis of 59 ribosomal proteins, including S14 was discerned. In every case, poly(A)+ mRNA programmed the cell-free system to synthesize in S14 protein electrophoretically identical to the S14 extracted from the ribosomes of the corresponding cell line. Messages from 2 Emt B mutants (Emr-2 and Emr-2-2) specified S14 that were electrophoretically distinct from the wild-type protein. Emt B mutations were expressed in mutant cell mRNA, apparently reflecting mutagen-induced changes in S14 structural genes.