2-Arachidonoylglycerol, an endogenous cannabinoid receptor ligand, induces the migration of EoL-1 human eosinophilic leukemia cells and human peripheral blood eosinophils
Open Access
- 17 August 2004
- journal article
- extracellular mediators-and-effector-molecules
- Published by Oxford University Press (OUP) in Journal of Leukocyte Biology
- Vol. 76 (5) , 1002-1009
- https://doi.org/10.1189/jlb.0404252
Abstract
2-Arachidonoylglycerol (2-AG) is an endogenous cannabinoid receptor ligand. To date, two types of cannabinoid receptors have been identified: the CB1 receptor, abundantly expressed in the brain, and the CB2 receptor, expressed in various lymphoid tissues such as the spleen. The CB1 receptor has been assumed to play an important role in the regulation of synaptic transmission, whereas the physiological roles of the CB2 receptor remain obscure. In this study, we examined whether the CB2 receptor is present in human eosinophils and found that the CB2 receptor is expressed in human peripheral blood eosinophils. In contrast, human neutrophils do not contain a significant amount of the CB2 receptor. We then examined the effect of 2-AG on the motility of eosinophils. We found that 2-AG induces the migration of human eosinophilic leukemia EoL-1 cells. The migration evoked by 2-AG was abolished in the presence of SR144528, a CB2 receptor antagonist, or by pretreatment of the cells with pertussis toxin, suggesting that the CB2 receptor and Gi/o are involved in the 2-AG-induced migration. The migration of EoL-1 cells induced by 2-AG was suggested to be a result of chemotaxis. In contrast to 2-AG, neither anandamide nor free arachidonic acid elicited the migration. Finally, we examined the effect of 2-AG on human peripheral blood eosinophils and neutrophils and found that 2-AG induces migration of eosinophils but not neutrophils. These results suggest that the CB2 receptor and its endogenous ligand 2-AG may be closely involved in allergic inflammation accompanied by the infiltration of eosinophils.Keywords
This publication has 48 references indexed in Scilit:
- Endocannabinoids in the immune system and cancerProstaglandins, Leukotrienes & Essential Fatty Acids, 2002
- Despite substantial degradation, 2‐arachidonoylglycerol is a potent full efficacy agonist mediating CB1 receptor‐dependent G‐protein activation in rat cerebellar membranesBritish Journal of Pharmacology, 2001
- Activation of PAF receptors results in enhanced synthesis of 2‐arachidonoylglycerol (2‐AG) in immune cellsThe FASEB Journal, 2001
- 2-Arachidonoylglycerol, a Putative Endogenous Cannabinoid Receptor Ligand, Induces Rapid, Transient Elevation of Intracellular Free Ca2+in Neuroblastoma × Glioma Hybrid NG108-15 CellsBiochemical and Biophysical Research Communications, 1996
- The peripheral cannabinoid receptor: adenylate cyclase inhibition and G protein couplingFEBS Letters, 1995
- Expression of Central and Peripheral Cannabinoid Receptors in Human Immune Tissues and Leukocyte SubpopulationsEuropean Journal of Biochemistry, 1995
- Molecular characterization of a peripheral receptor for cannabinoidsNature, 1993
- Isolation and Structure of a Brain Constituent That Binds to the Cannabinoid ReceptorScience, 1992
- Structure of a cannabinoid receptor and functional expression of the cloned cDNANature, 1990
- Differential Sensitivities of Purified Human Eosinophils and Neutrophils to Defined ChemotaxinsScandinavian Journal of Immunology, 1989