Isolation and Characterization of a Homogeneous DNA-Protein Complex from Adenovirus Type 2 Virion

Publisher Website
Google Scholar
Abstract
Adenovirus DNA-protein complex purified by sedimentation on a sucrose gradient containing 4 M-guanidine hydrochloride contained other virion proteins in addition to the terminal protein of MW 55,000. A simple and rapid method for the isolation of a homogeneous DNA-protein complex is described. The procedure involves gel electrophoresis of the complex on agarose in the presence of sodium dodecyl sulfate. DNA migrated into the gel with a single protein of MW 55,000 tightly attached to it. Restriction enzyme cleavage analysis of the DNA-protein complex shows that the protein is associated with the 2 terminal fragments.