Sperm motility in the horseshoe crab. III. Isolation and characterization of a sperm motility initiating peptide
- 1 January 1982
- journal article
- research article
- Published by Wiley in Gamete Research
- Vol. 6 (4) , 315-326
- https://doi.org/10.1002/mrd.1120060404
Abstract
Sperm motility in Limulus is initiated by a sperm motility initiating factor (SMI) that emanates from Limulus eggs. This report describes the partial purification of SMI (greater than 230‐fold purification with respect to protein content) with 40% recovery. SMI appears to be a hydrophobic peptide of 500–2,000 MW. Although probably not purified to homogeneity, SMI is estimated to be active at a concentration of less than 0.2 μM.Keywords
This publication has 10 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Purification and the primary structure of sperm-activating peptides from the jelly coat of sea urchin eggsBiochemical and Biophysical Research Communications, 1981
- Speract. Purification and characterization of a peptide associated with eggs that activates spermatozoa.Journal of Biological Chemistry, 1981
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnalytical Biochemistry, 1980
- Sperm motility in the horseshoe crab, Limulus polyphemus LDevelopmental Biology, 1980
- Sperm motility in the horseshoe crab, Limulus polyphemus LDevelopmental Biology, 1980
- Taurine maintains and stimulates motility of hamster sperm during capacitation in vitroJournal of Experimental Zoology, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970