Membrane-associated Glycosyl Transferases in Cotyledons of Pisum sativum

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Abstract
In crude particulate fractions isolated from pea (P. sativum) cotyledons, the transfer of radioactivity from GDP-[14C]mannose to glycolipid appears to be preferentially stimulated by Mn2+ while the transfer to lipid-free residue is enhanced by Mg2+. The transfer of radioactivity from UDP-N-acetyl-[14C]glucosamine to glycolipid shows preferential stimulation by Mg2+ while the transfer to lipid-free residue prefers Mn2+. These results are accounted for by the differential stimulation by Mg2+ and Mn2+ of glycosyl transferases associated with subcellular membranes which were separated by isopycnic sucrose density centrifugation.