Creatine Phosphokinase Isoenzymes in Stress-Susceptible and Stress-Resistant Pigs

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Abstract
Sixteen stress-resistant (SR) and 16 stress-susceptible (SS) pigs were used in the study. Eight SR and eight SS pigs were subjected to a 5-min stress 4 hr bef ore slaughter. The other 16 pigs were not stressed. A catheter was inserted into the vena cava of all pigs and blood samples were collected through the catheter before the stress period and 2 and 4 hr poststress. Blood samples from the nonstressed pigs were obtain-ed before slaughter. M. longissimus, Triceps brachii, ventricular myocardium and cerebral cortex tissue samples were obtained immediate-ly after exsanguination for creatine phospho-kinase (CPK) analysis. Total CPK and CPK isoenzymes were measured in duplicate for each sample. Serum CPK levels were significantly greater in the SS pigs as compared with SR pigs for all comparisons studied. Serum levels of the skeletal muscle CPK isoenzyme (MM-CPK) and the cardiac muscle CPK isoenzyme (MB-CPK) were significantly different between SS and SR pigs in the 2-hr poststress samples. Total CPK in the Triceps brachii and the ventricular myocardium was not significantly different when the SS and SR pigs were compared. Also, no significant differences were found in the CPK isoenzyme composition of the M. longissimus, Triceps brachii and cerebral cortex. Rested SS pigs had more total CPK in their M. longissimus than stressed SS pigs, and rested SS and SR pigs had more CPK in the cerebral cortex as compared with stressed SS and SR pigs. Stressed SS and SR pigs showed significantly more MM-CPK and MB-CPK in the heart (P<.05) than the rested SS and SR pigs. Copyright © 1979. American Society of Animal Science . Copyright 1979 by American Society of Animal Science

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