In Vitro Propagation of Japanese Plum (Prunus salicina Lindl. cv. Calita)1
Open Access
- 1 January 1980
- journal article
- Published by American Society for Horticultural Science in Journal of the American Society for Horticultural Science
- Vol. 105 (1) , 126-129
- https://doi.org/10.21273/jashs.105.1.126
Abstract
Tissue cultures of ‘Calita’ Japanese plum started from 5 to 7 mm long shoot tips proliferated at a rate of 10:1 to 20:1 per month when grown on a modified Murashige and Skoog medium with 3% sucrose, 0.75% agar and (in mg/liter) 0.4 thiamine HCl, 100 myo-inositol, 1.0 6-benzylamino purine (BA), 0.1 gibberellic acid (GA3) and 0.1 indolebutyric acid (IBA). Best rooting results were obtained with 2 or 4 mg/liter indolebutyric acid (IBA) at 21°C; raising the temperature to 26° or 30° slowed and decreased rooting unless 0.1 mg/liter GA3 was included in the medium. Activated charcoal in the medium drastically reduced rooting. Rooted cuttings were easily established in soil.Keywords
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