A simple fixation and embedding method for use in hybridization histochemistry on plant tissues
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 20 (10) , 575-586
- https://doi.org/10.1007/bf01002612
Abstract
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acidsin situ. Double-stranded32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron nucroscopic level.This publication has 32 references indexed in Scilit:
- Hybridization histochemistryCellular and Molecular Life Sciences, 1987
- Usefulness of the immunogold technique in quantitation of a soluble protein in ultra-thin sections.Journal of Histochemistry & Cytochemistry, 1987
- Developmentally controlled expression of a gene associated with self-incompatibility in Nicotiana alataNature, 1987
- Letter to the editorJournal of Molecular Histology, 1987
- Some New Methods for Affixing Sections to Glass Slides. I. Aqueous AdhesivesStain Technology, 1987
- In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probesJournal of Molecular Histology, 1986
- Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alataNature, 1986
- Efficiency of in situ hybridization as a function of probe size and fixation techniqueJournal of Virological Methods, 1985
- Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.Journal of Histochemistry & Cytochemistry, 1983
- Phase-Partition Fixation and Staining ofDrosophzlaEggsStain Technology, 1977