Participation of the Human β-Globin Locus Control Region in Initiation of DNA Replication
- 3 November 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 270 (5237) , 815-819
- https://doi.org/10.1126/science.270.5237.815
Abstract
The human β-globin locus control region (LCR) controls the transcription, chromatin structure, and replication timing of the entire locus. DNA replication was found to initiate in a transcription-independent manner within a region located 50 kilobases downstream of the LCR in human, mouse, and chicken cells containing the entire human β-globin locus. However, DNA replication did not initiate within a deletion mutant locus lacking the sequences that encompass the LCR. This mutant locus replicated in the 3′ to 5′ direction. Thus, interactions between distantly separated sequences can be required for replication initiation, and factors mediating this interaction appear to be conserved in evolution.Keywords
This publication has 30 references indexed in Scilit:
- On the nature of replication origins in higher eukaryotesCurrent Opinion in Genetics & Development, 1995
- Intergenic DNA and the sequence requirements for replication initiation in eukaryotesCurrent Opinion in Genetics & Development, 1994
- Replication structure of the human & beta;-globin gene domainNature, 1993
- Developmental regulation of human β-globin gene transcription: a switch of loyalties?Trends in Genetics, 1993
- How transcription factors regulate origins of DNA replication in eukaryotic cellsTrends in Cell Biology, 1993
- Transcriptional regulation of multigene loci: multilevel controlTrends in Genetics, 1993
- Introduction of YACs containing a putative mammalian replication origin into mammalian cells can generate structures that replicate autonomouslySomatic Cell and Molecular Genetics, 1993
- Regulation of the β-globin locusCurrent Opinion in Genetics & Development, 1993
- Mapping replication units in animal cellsCell, 1989
- Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 1987