SEROLOGICAL AND CHEMICAL STUDIES ON ESCHERICHIA COLI RNA PHAGES

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Abstract
Four RNA phages, [beta], Bl, and I isolated here, and MS2 isolated in the United States were studied. At 37[degree], plaque types of Bl and I were larger than those of [beta] and MS2. At 45[degree], Bl and I gave pin-hole-type plaques and [beta] and MS2 medium size plaques which were as large as those at 37[degree]. MS2 infected bacteria began to lyse in 45 min after infection and [beta] infected bacteria in 55-60 min. The bacteria infected with Bl or I lysed to a negligible extent. The 4 pahges were serologlcally related and arranged in an order of [beta], MS2, Bl, I by t e the neutralization experiment. Base composition of phage RNAs were studied. In MS2 RNA and [beta] RNA, M/% of guanylic acid was larger than that of urldyllc acid, and in Bl RNA and I RNA, M/% of uridylic acid was larger than that of guanylic acid. Hybridization experiments were conducted between a double stranded RNA of [beta] and a single stranded RNA of other phages. The results suggest that the [beta] RNA was highly homologous with MS2 RNA, and to lesser extents with Bl RNA and I RNA. Correlation between the serological character and the RNA structure is discussed.

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