LORE1, an active low‐copy‐number TY3‐gypsy retrotransposon family in the model legume Lotus japonicus
- 7 October 2005
- journal article
- Published by Wiley in The Plant Journal
- Vol. 44 (3) , 372-381
- https://doi.org/10.1111/j.1365-313x.2005.02534.x
Abstract
We have identified a low-copy-number retrotransposon family present in nine to 10 copies in the Lotus japonicus model legume genome, and characterized its activity. LORE1 (Lotus retrotransposon 1) belongs to the Ty3-gypsy group of elements, and is a long terminal repeat (LTR) retrotransposon. Genetic mapping located LORE1 elements in gene-rich regions of Lotus chromosomes, and analysis of native as well as new insertion sites revealed integration outside the highly repetitive sequences of centromeres and telomeres. Sequencing of individual LORE1 family members identified several intact elements, and analysis of new insertions showed that at least one member is active and reinserts into functional genes, creating gene-disruption mutations. Southern blot analysis and SSAP on a selection of symbiotic mutants revealed up to 12 new insertion sites in individual mutant lines and a Mendelian segregation of new inserts. Expression analysis showed that LORE1 elements are transcribed in all organs analysed and, in contrast to other active retrotransposons, LORE1 appears not to be transcriptionally upregulated during in vitro tissue culture. Activity of LORE1 in callus and whole plants suggests that a simple insertion mutagenesis based on endogenous LORE1 elements can be established for Lotus.Keywords
This publication has 54 references indexed in Scilit:
- The Novel Gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of Rice Encodes a Putative Coiled-Coil Protein Required for Homologous Chromosome Pairing in MeiosisPlant Cell, 2004
- An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosisMolecular Genetics and Genomics, 2004
- Plant recognition of symbiotic bacteria requires two LysM receptor-like kinasesNature, 2003
- Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary WallPlant Physiology, 2003
- Target Site Specificity of the Tos17 Retrotransposon Shows a Preference for Insertion within Genes and against Insertion in Retrotransposon-Rich Regions of the GenomePlant Cell, 2003
- Two-Step Regulation and Continuous Retrotransposition of the Rice LINE-Type Retrotransposon KarmaPlant Cell, 2003
- Isolation and Characterization of Rice Phytochrome A MutantsPlant Cell, 2001
- Genetic distribution of Bare–1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP)Molecular Genetics and Genomics, 1997
- Autonomous transposition of the tobacco retrotransposon Tto1 in rice.Plant Cell, 1996
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995