Salmonella typhimurium metC operator-constitutive mutations
- 15 July 1989
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 60 (2) , 137-141
- https://doi.org/10.1016/0378-1097(89)90496-5
Abstract
We used an Escherichia coli lac deletion strain lysogenized with a metC-lacZ fusion phage (λClac) to select operator-constitutive mutations in the Salmonella typhimurium metC gene control region. The mutations were located in a region containing 2 tandemly repeated 8 bp palindromes previously proposed to be the MetJ repressor binding site. Lysogens carrying λClac mutant phage exhibit high β-galactosidase levels that are only partially repressible by methionine. The results suggest that the mutations disrupt the methionine control system mediated by the metJ gene product.Keywords
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