ESTIMATION IN VIVO OF THE VIABILITY OF FROZEN AND STORED BONE MARROW

Publisher Website
Google Scholar
Abstract
The loss of viability of frozen mouse bone marrow has been estimated in three ways after injection into irradiated mice; firstly, by determination of its potential as a therapeutic agent in affecting survival; secondly, by determination of its capacity to restore various peripheral blood components towards normal; and thirdly, by determination of its capacity to give rise to spleen spots. These estimates are compared and discussed. Freezing was found to diminish the therapeutic potential by 90%, the capacity to restore peripheral blood components by 50% (little difference between the various components was observed), and the potential for giving rise to spleen spots by 50%. The possibility that frozen marrow may not be able to effect haematopoiesis at normal levels for more than about 10 days is considered.