Augmentation of Killing of Escherichia coli O157 by Combinations of Lactate, Ethanol, and Low-pH Conditions
- 1 March 1999
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 65 (3) , 1308-1311
- https://doi.org/10.1128/aem.65.3.1308-1311.1999
Abstract
The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.Keywords
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