Vital Staining of Blood Parasites with Acridine Orange

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Abstract
Vital staining of the cellular elements of normal and parasitized whole blood with the basic fluorochrome acridine orange was shown to be a rapid and simple technique for examining, under dark-field illumination, differentiated leucocytes, and stained parasitized erythrocytes, containing the malarial organism in the presence of unstained normal erythrocytes. Microfilariae, trypanosomes and Toxoplasma have also been stained in whole blood smears. Auxiliary use of glass filters has shown that the disadvantages of thick blood suspensions may be eliminated, permitting rapid observation of leucocytes and blood parasites. Photochemical inactivation of leptospires and microfilariae is demonstrated. Differences in sensitivity or tolerance to acridine orange were found in T. cruzi and T. equiperdum.

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