Antibody Production in Silkworm Cells and Silkworm Larvae Infected with a Dual Recombinant Bombyx Mori Nuclear Polyhedrosis Virus
- 1 August 1992
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 10 (8) , 910-912
- https://doi.org/10.1038/nbt0892-910
Abstract
We have examined the efficiency of coexpression of two heterologous genes from a recombinant Bombyx mori nuclear polyhedrosis virus for the production of antibodies in silkworm larvae. The cDNAs encoding the light and the heavy chains of a murine immunoglobulin, directed against lipoprotein I of Pseudomonas aeruginosa, were brought under the control of two separate copies of the viral polyhedrin promotor. Infection of silkworm cells with the recombinant baculovirus yielded a maximum of 6.4 micrograms/ml IgG2A in the culture supernatant 72 hours post infection, while 800 micrograms/ml IgG2A was found in the hemolymph of infected fifth instar silkworm larvae seven days after infection with the same construct. The recombinant antibody exhibited a similar antigen specificity and avidity to that of the monoclonal antibody derived from ascites fluid.Keywords
This publication has 25 references indexed in Scilit:
- Gene Transfer Vectors of a Baculovirus, Bombyx Mori Nuclear Polyhedrosis Virus, and Their use for Expression of Foreign Genes in Insect CellsPublished by Taylor & Francis ,2018
- Assembly of five bluetongue virus proteins expressed by recombinant baculoviruses: Inclusion of the largest protein VP1 in the core and virus-like particlesVirology, 1991
- High-level production of a functional immunoglobulin heterodimer in a baculovirus expression system.Proceedings of the National Academy of Sciences, 1990
- Insect baculoviruses: Powerful gene expression vectorsBioEssays, 1989
- Expression of eucaryotic genes in insect cell culturesIn Vitro Cellular & Developmental Biology, 1989
- Insect cell culture technology in baculovirus expression systemsTrends in Biotechnology, 1989
- Baculoviruses as Gene Expression VectorsAnnual Review of Microbiology, 1988
- Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3Gene, 1987
- High-level expression of the human-.ALPHA.-interferon gene through the use of an improved baculovirus vector in the silkworm, Bombyx mori.Agricultural and Biological Chemistry, 1987
- Production of human α-interferon in silkworm using a baculovirus vectorNature, 1985