SURFACE-ANTIGEN CHANGES ACCOMPANYING LYMPHOCYTE-ACTIVATION - SELECTIVE REACTIVITY OF CERTAIN ANTI-H-2 ANTISERA WITH ACTIVATED LYMPHOCYTES OF UNRELATED H-2 HAPLOTYPE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 6  (10) , 1029-1040
Abstract
Some noncongenic anti-H-2 antisera produced by using normal [mouse] spleen cells as the immunogen can contain cytotoxic antibody activity against activated, but not resting, lymphocytes of unrelated H-2 haplotype strains. Reactivity was noted against lymphocytes activated in vitro with T[thymus-derived]- or B[bone marrow derived]-cell mitogens, or in vivo by antigenic stimulation. The anti-activated lymphocyte activity was most likely related to production of anti-Ala-1 (activated lymphocyte antigen) alloantibodies. This appears to occur as a result of donor lymphocyte activation during production of the anti-H-2 antisera, using Ala-1-incompatible donor and recipient combinations where the donor cells are competent to recognize and react against host H-2 incompatibilities. Lymphocyte activation can be accompanied by the expression of an antigen that is either absent or weakly expressed on resting lymphocytes. [Guinea pig complement was used.].