RADIOMETRIC STUDIES OF MYCOBACTERIUM-LEPRAEMURIUM

  • 1 January 1976
    • journal article
    • research article
    • Vol. 44  (3) , 294-300
Abstract
The radiometric method was applied for studying the metabolism of M. lepraemurium and the conditions which might force or inhibit its metabolic activity in vitro. These organisms assimilate and oxidize (U-[uniformly labeled]14C) glycerol and (U-14C) acetate, but are unable to oxidize (U-14C) glucose, (U-14C) pyruvate (U-14C) glycine and 14C-formate. When incubated at 30.degree. C M. lepraemurium oxidizes (U-14C) acetate to 14CO2 faster than at 37.degree. C. The same effect was observed with increasing concentrations of polysorbate 80 (Tween 80), or the 14C-substrate. No change in metabolic rate was observed when the organisms were kept at -20.degree. C for 12 days. Although tried several times, it was not possible to demonstrate any inhibitors of bacterial metabolism in the reaction system. The radiometric method seems to be an important tool for studying metabolic pathways and the influence of physical and biochemical factors on the metabolism of M. lepraemurium in vitro.

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