HISTOCHEMICAL STUDIES ON ACHD MUCOPOLYSACCHARIDES

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Abstract
Summary: Some new methods for demonstration and differentiation of acid mucopolysaccharides were described. Cationic surfactive resin‐azo dye method could be used for all kinds of acid mucopolysaccharide demonstration. Neutral red method was found to differentiate sulfated and non‐sulfated groups on one section. Two principles to identify keratosulfate in mesenchymal tissues were studied; Hyaluronidase‐methylation‐saponification method can differentiate keratosulfate B which are both sensitive to testicular hyaluronidase. Sugar reactions: Molisch reaction, carbazol‐sulfuric acid reaction, etc. can demonstrate the presence of galactose‐containing keratosulfate in the frozen sections. By using these together with well established methods a differential table was made.The author is deeply grateful to Dr. SHIDA and Dr. KUSHIDA of the General Institute for Medical Biochemistry, Kyoto, Kowa Co., Kaken Yakukako Co., and Hodogaya Chemical Industry Co. for the supply of several kinds of samples.