TUBULORETICULAR STRUCTURES IN HUMAN LYMPHOID-CELL LINES - CELL BIOLOGICAL STUDY

  • 1 January 1975
    • journal article
    • research article
    • Vol. 56  (2) , 124-132
Abstract
Human lymphoid cell lines were studied as an experimentel model for the spontaneous or induced occurrence of tubuloreticular structures (TRS). It was possible to induce TRS after culturing the [human Burkitt lymphoma] EB-3 cell line with 20 .mu.g/ml bromodeoxyuridine (BrUdR) for 96 h. Starvation, culturing at lower temperature (32.degree. C) or inhibition of DNA synthesis did not give rise to the production of TRS. The response to BrUdR could be blocked with 60 .mu.g/ml thymidine but not with 60 .mu.g/ml deoxycytidine. The addition of 5 .mu.g/ml cytarabine or the removal of BrUdR at different times resulted in inhibition of TRS induction, indicating that BrUdR had to be incorporated into DNA for at least 48 h. After incorporation, the presence of BrUdR or DNA synthesis was not necessary for the production of TRS. These experiments and the finding that in the cell line IHTC-33, which does not produce Epstein-Barr virus associated antigens. TRS were spontaneously present, exclude a correlation between TRS and these antigens. The induction of TRS by BrUdR may be related to the activation of another (latent) virus.