Reversed-Phase High Performance Liquid Chromatographic Determination of Nifedipine in Human Plasma

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Abstract
A reversed-phase high performance liquid chromatographic method is presented by which the Ca channel blocker, nifedipine (NFP), may be measured in human serum using 17-.alpha.-ethinylestradiol as an internal standard. A mobile phase of phosphate buffer (0.01 M, pH 6.1)/methanol/acetonitrile (20:35:45) passed through a .mu.Bondapak C-18 column at 1.0 ml/min produced symmetrical bands for nifedipine and interal standard. A rapid and simple chloroform extraction of NFP from 1 ml serum samples proved to be efficient and reproducible. Recovery over a concentration range of 5-100 ng/ml was 92.3 .+-. 5.1% (mean .+-. SD, n = 6). UV absorbance detection at 235 nm was sensitive to serum NFP concentrations of 5 ng/ml. Between-day coefficients of variation at 100 and 5 ng/ml were 4.0 and 11.4%, respectively (n = 10). Serum concentration data from NFP-treated subjects are presented.