Allergoprints in Horse Allergic Children

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Abstract
To determine the allergenic activity of 5 purified horse allergens, 22 children allergic to horses according to history, skin test and leukocyte histamine release were evaluated. Washed leukocytes from all patients were tested for allergen-induced histamine release utilizing 4 epidermal horse allergens (Ag 6, 9, 11 and 15) and horse serum albumin. Crossed radioimmunoelectrophoresis was carried out with a standardized horse dander extract and serum from each patient. Results showed considerable variation in the individual allergoprints. Ag 11 had the highest mean allergenic activity. Sensitivity to horse serum albumin was demonstrated 3 times. The amount of serum IgE antibodies bound by horse allergens correlates significantly with the capacity of the allergens to induce histamine release from washed leukocytes.