Selective labeling of mesenteric lymph nodes: Cell production and emigration ofnewly formed lymphocytes to other organs
- 1 October 1979
- journal article
- research article
- Published by Wiley in The Anatomical Record
- Vol. 195 (2) , 341-355
- https://doi.org/10.1002/ar.1091950208
Abstract
Lymphocyte production by mesenteric lymph nodes of normal young pigs was studied by intranodal injections of either tritiated thymidine or tritiated deoxycytidine as DNA precursors. One or two days after selective labeling of the mesenteric lymph nodes the relative and absolute number of lymphocytes derived from mesenteric lymph nodes were determined autoradiographically in the following organs: mesenteric, cervical and inguinal lymph nodes, spleen, thymus, bone marrow, peyer'spatches, tonsil, different regions of the gut, lung and liver. The overall cell production of mesenteric lymph nodes, as derived from the sum of all labeled cells one day after labeling, was estimated to be about 7 × 109 lymphocytes. Up to 40% of all newly formed lymphocytes had already left the lymph nodes within one day and were found in all organs studied. There was a preferential homing to the mucosa of the small intestine, but a considerable number migratedto the spleen and even to the thymus and bone marrow. In lymphoid organs all labeled cells were small and medium‐sized lymphocytes one and two days after labeling. In cervical lymph nodes, spleen, tonsil and peyer's patches the relative distribution to T and B cell areas was determined. There was an obvious preference of newly formed lymph node cells to home to T cell areas. The differences of labeling between thymidine or deoxycytidine were surprisingly low.This publication has 15 references indexed in Scilit:
- Evaluation of lymphocyte production in mesenteric lymph nodesZeitschrift für Die Gesamte Experimentelle Medizin, 1979
- Splenic lymphocytopoiesis and migration pattern of splenic lymphocytesCellular Immunology, 1977
- Studies on the lymphocytes of sheep. Destination of lymph‐borne immunoblasts in relation to their tissue of originEuropean Journal of Immunology, 1977
- Selective entry of immunoblasts into gut from intestinal lymphNature, 1976
- The gut-associated lymphoid tissues and gastrointestinal immunityPublished by Springer Nature ,1976
- Quantitative evaluation of the total number and distribution of lymphocytes in young pigsAnnals of Hematology, 1975
- Estimation of the proportion of cell nuclei in tissue sections falling within tritium-autoradiographic rangeExperimental Cell Research, 1973
- Migration of bone marrow lymphocytes demonstrated by selective bone marrow labeling with thymidine-H3The Anatomical Record, 1970
- THE DISTRIBUTION OF LARGE DIVIDING LYMPH NODE CELLS IN SYNGENEIC RECIPIENT RATS AFTER INTRAVENOUS INJECTIONThe Journal of Experimental Medicine, 1969
- Migration of Cells to the Thymus Demonstrated by Parabiosis.Experimental Biology and Medicine, 1967