Effect of Lipid Materials on the Production of Lipase by Torulopsis ernobii
Open Access
- 1 January 1968
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 16 (6) , 845-847
- https://doi.org/10.1128/aem.16.6.845-847.1968
Abstract
The yeast Torulopsis ernobii produced approximately 50 units of extracellular lipase (glycerol ester hydrolase, E.C. 3.1.1.3) per ml when grown in 30-liter fermentors at 33 C for 40 hr in a base medium at pH 5.0. The addition of fats, oils, triglycerides, or higher fatty acids to this medium at concentrations of 0.2 to 0.6% markedly increased production; twofold increases in yield were obtained when 0.2% olive oil or a mixture of 0.14% oleic acid and 0.04% palmitic acid was added. The production of lipase paralleled growth, and the role of lipid materials in augmenting lipase production appears to be related to cell growth.Keywords
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