Identification of Monoterpene Alcohol .BETA.-Glucosides in Sweet Potatoes and Purification of a Shiro-koji .BETA.-Glucosidase.
- 1 January 1991
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 55 (7) , 1811-1816
- https://doi.org/10.1271/bbb1961.55.1811
Abstract
Linalyl-β-glucoside (LBG), α-terpinyl-β-glucoside (TBG), neryl-β-glucoside (NBG), and geranyl-β-glucoside (GBG) in sweet potatoes were identified as TMS derivatives from their MS fragmentation patterns and retention times. Amounts of these monoterpene alcohol-β-glucosides were from 36.9 μg/kg sweet potato of LBG to 189.μg/kg sweet potato of TBG. On the other hand, 75.8μg/1 distillation residue of TBG and traces of other monoterpene alcohol-β-glucosides occurred in the distillation residue. β-glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) in shiro-koji were purified using p-nitrophenyl-β-glucoside (PNPG), and three active fractions, one major fraction and two very minor fractions, were found. The Km against GBB was 2.4 mM and the Ki against glucose was 7.6 mM. Relative activity in the 20% ethanol solution was 68%. Shiro-koji β-glucosidases were active on tested β-glucosides except for LBG and TBG.Keywords
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