DURATION OF MOTILITY AND GLYCOLYSIS OF FOWL SPERMATOZOA IN VITRO UNDER ANAEROBIC CONDITIONS, CONSTANT pH AND CONSTANT GLUCOSE CONCENTRATION

Publisher Website
Google Scholar
Abstract
Duration of motility and glycolysis, total acid and lactic acid production as a function of time were determined in fowl spermatozoa in vitro, under anaerobic conditions, with constant pH, glucose concentration and temperature. A constant pH (7.1) was maintained without the use of buffer by an automatic titrator, which also provided a continuous record of rate and quantity of acid production by the spermatozoa. During the first few hours of incubation both motility and metabolic rate declined gradually and then stabilized at a constant value. Motility remained constant for a period of 40 to 50 hr and then declined to zero within a few hours. No change in the rate of acid production took place during the final decline of motility. It remained constant till the termination of the experiment 20 hr later and was associated exclusively with the spermatozoa. Cessation of motility was not caused by the accumulation of toxic substances in the medium.