REFRACTOMETRY AS A MEASURE OF IMMUNOGLOBULIN STATUS OF NEWBORN DAIRY CALF - COMPARISON WITH ZINC-SULFATE TURBIDITY TEST AND SINGLE RADIAL IMMUNODIFFUSION

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (9) , 1331-1334
Abstract
Immunoglobulins [Ig] were quantitated by single radial immunodiffusion in 34 female Holstein-Friesian calves which were kept with their dams for the first 24 h of life. The mean IgG1 concentration was 1.063 g/dl; IgG2, 0.093 g/dl; IgM, 0.171 g/dl; and IgA, 0.125 g/dl. Serum total protein as measured by refractometer and ZnSO4 turbidity measured at 498 nm gave good correlations with total protein, which were significant (P < 0.001). Plasma total protein had a slightly poorer correlation with total Ig, presumably due to variable fibrinogen content. Plasma total protein gave a better correlation with total Ig than did any of the Ig classes individually. Total protein by refractometer underestimated naturally occurring or added Ig by 1/3.

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