Biochemical and Physiological Studies of Certain Ticks (Ixodoidea). Excretion during ixodid feeding12

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Abstract
Excreta from females of 9 ixodid species in the genera Hyalomma, Dermacentor, and Haemaphysalis, and from nymphal Dermacentor andersoni, contained 1.4–9.3% guanine, 2.9–15.0% “2nd purine,” 0.19–1.5% hematin, and 5.0–68.0% protein by weight. Polyacrylamide gel electrophoresis of excreta showed only hemoglobin and albumin. The quantity of protein in D. andersoni excreta differed when the ticks fed on different hosts. Thin-layer chromatography showed 1–9 ninhydrin-positive spots for each tick species; these were identified as arginine, serine, alanine, histidine, valine, glutamate, aspartate, cystcinc, glycine, and phenylalanine.

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